The Aristotle University of Thessaloniki, School of Veterinary Medicine, recently hosted a highly successful training school from September 23-25, 2024, focusing on Next Generation Sequencing (NGS) using Nanopore Technology for the diagnosis of swine influenza A viruses (swIAV). This event was part of the networking and training activities organized under COST Action CA 21132 (ESFLU), aiming to foster collaboration and knowledge exchange across Europe. During the three-day event, 12 young scientists and lab technicians from various institutions from a total of 9 European countries received hands-on training in the use of Nanopore sequencing technology. This advanced technique represents a major leap forward in the molecular diagnosis of swine influenza, a disease that continues to threaten both animal and human health worldwide.

The training school offered a dynamic mix of theoretical lectures and practical workshops. Participants engaged in practical exercises on sample preparation, data generation, and bioinformatics analysis using the latest Nanopore technology. These sessions were complemented by expert-led discussions and exchange of experience on how this technology can be used for real-time surveillance and monitoring of swIAV mutations and spread.
Led by the staff of the Laboratory of Microbiology and Infectious diseases of the Aristotle University and WG1 representatives, the training provided participants with an in-depth understanding of the applications of NGS in veterinary virology. The program emphasized the use of Nanopore sequencing for rapid, accurate virus identification and tracking, a critical tool in modern veterinary diagnostics. Main objective of the WG1 is to Strengthen the capability in Europe for the identification and characterisation of swIAV.
This training school marks a significant achievement in the professional development of early-career scientists, providing them with cutting-edge skills that are critical for addressing current and emerging challenges in swine influenza diagnosis. Participants left the event well-prepared to contribute to enhanced disease diagnosis, surveillance and control efforts in their respective countries.
As part of COST Action CA 21132, this event underscores the importance of interdisciplinary collaboration and capacity building in combating infectious diseases across Europe.

