New ESFLU Guidance: Laboratory Protocols for the Detection and Monitoring of Swine Influenza A Virus
ESFLU is pleased to announce the publication of its new guidance document, Addressing Swine Influenza A Virus – Protocols and Guidelines for Laboratory Diagnosis.
With the increasing circulation of swine influenza viruses in European pig populations and the emergence of new variants, including strains with zoonotic potential, robust surveillance and monitoring are more important than ever. This guidance provides example molecular, serological, and virological protocols for the detection and monitoring of swine influenza A virus (IAV-S).
The document aims to support harmonized laboratory practices while recognizing that laboratories may use alternative validated methods. The protocols are provided as examples and do not constitute an endorsement of any specific company or commercial product.
Developed through the collaborative efforts of the ESFLU working group 1, this guidance is intended to support improved surveillance, knowledge exchange, and coordinated responses to emerging swine influenza threats.
Acknowledgements
This publication is based upon work from COST Action CA21132, European Swine Influenza Network (ESFLU) supported by COST (European Cooperation in Science and Technology). COST is a funding agency for research and innovation networks. Our Actions help connect research initiatives across Europe and enable scientists to grow their ideas by sharing them with their peers. This boosts their research, career and innovation. For more information, visit the COST website.
We gratefully acknowledge the ESFLU consortium members for sharing their SOPs and providing valuable input that supported the development of this guideline, in particular:
Gwenaëlle DAUPHIN, Ceva Santé Animale, Action Chair of the COST Action ESFLU
Timm Harder, Friedrich-Loeffler-Institute, Greifswald – Insel Riems, Germany
Jacqueline King, Friedrich-Loeffler-Institute, Greifswald – Insel Riems, Germany
Anne Pohlmann, Friedrich-Loeffler-Institute, Greifswald – Insel Riems, Germany
Jesper Schak Krog, Statens Serum Institut, Denmark
Gaelle Simon, Agence Nationale de la Securité Sanitaire de l’Alimentation de l’Environnement et du Travail, France
Séverine HERVE, Agence Nationale de la Securité Sanitaire de l’Alimentation de l’Environnement et du Travail, France
Celine Deblanc, Agence Nationale de la Securité Sanitaire de l’Alimentation de l’Environnement et du Travail, France
Lara Cavicchio, Istituto Zooprofilattico Sperimentale Delle Venezie, Italy
Letizia Ceglie, Istituto Zooprofilattico Sperimentale Delle Venezie, Italy
Jwee Chiek Er, Norwegian Veterinary Institution – Norwegian Veterinary Institute, Norway
Carl Andreas Grontvedt, The Norwegian Veterinary Institute, Norway
Tuija Gadd, Finnish Food Authority, Finland
Jonna Kyyrö, Finnish Food Authority, Finland
Riikka Kaarto, Finnish Food Authority, Finland
Authors:
Annika Graaf-Rau, Department of Pathogen Evolution, Helmholtz-Institute for One Health (HIOH), Greifswald, Germany
Chiara Chiapponi, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia-Romagna (IZSLER), Brescia, Italy
Helen Everett, Department of Virology, Animal and Plant Health Agency (APHA), Woodham Lane, New Haw, Addlestone, Surrey, UK
Charlotte Kristiane Hjulsager, Department for Virology and Microbiological Preparedness, Statens Serum Institut, 2300 Copenhagen S, Denmark
Alice Prosperi, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia-Romagna (IZSLER), Brescia, Italy
Ada Mescoli, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia-Romagna (IZSLER), Brescia, Italy
Ana Maria Moreno, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia-Romagna (IZSLER), Brescia, Italy
Sasan Fereidouni, Research Institute of Wildlife Ecology, University of Veterinary Medicine Vienna, Vienna, Austria
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